DMEM/F-12

Price range: $27.08 through $36.48

DMEM/F12, a 1:1 mixture combining the benefits of DMEM with the added nutrients found in Ham’s F-12, and is widely used for supporting the growth of many different mammalian cells including MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts.

Catalog#	Product Name/Description	Volume	Price	BuySelect all
18101051	DMEM/F-12 - DMEM/F12 (1:1), 500 ml, Without L glutamine , With HEPES	500 ml	$27.28	DMEM/F-12 quantity
18101053	DMEM/F-12 - DMEM/F12 (1:1), 500 ml, With L glutamine , Without HEPES	500 ml	$27.08	DMEM/F-12 quantity
18101055	DMEM/F-12 - DMEM/F12 (1:1), 500 ml, With L glutamine , With HEPES	500 ml	$27.18	DMEM/F-12 quantity
18101057	DMEM/F-12 - DMEM/F12 (1:1), 500 ml, With L glutamine , With HEPES, With sodium pyruvate	500 ml	$27.18	DMEM/F-12 quantity
18101052	DMEM/F-12 - DMEM/F12 (1:1), 1000 ml, Without L glutamine , With HEPES	1000 ml	$35.98	DMEM/F-12 quantity
18101054	DMEM/F-12 - DMEM/F12 (1:1), 1000 ml, With L glutamine , Without HEPES	1000 ml	$36.18	DMEM/F-12 quantity
18101056	DMEM/F-12 - DMEM/F12 (1:1), 1000 ml, With L glutamine , With HEPES	1000 ml	$36.28	DMEM/F-12 quantity
18101058	DMEM/F-12 - DMEM/F12 (1:1), 1000 ml, With L glutamine , With HEPES, With sodium pyruvate	1000 ml	$36.48	DMEM/F-12 quantity

Overview

Dulbecco’s Modified Eagle Medium Nutrient Mixture F-12 (DMEM F12), a 1:1 mixture combining the benefits of DMEM with the added nutrients found in Ham’s F-12. This formulation combines DMEM’s high concentrations of glucose, amino acids, and vitamins with the wide variety of components found in Ham’s F12 media. VitaCellix™ DMEM/F12 is a widely used basal medium for supporting the growth of many different mammalian cells including MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. VitaCellix™ DMEM/F12 is formulated for superior quality and delivers greater reliability, consistency, and improved control in mammalian cell culture. Along with the glucose, amino acids, and vitamins in DMEM, DMEM/F12 contains additional components found in Ham’s F12 media, including zinc, putrescine, hypoxanthine, and thymidine. DMEM/F12 may also include other factors such as L-glutamine and phenol red indicator. However, due to its lack of proteins, lipids, and growth factors, DMEM/F12 is commonly supplemented with fetal bovine serum (FBS). In addition, the sodium bicarbonate buffer system used in DMEM F12 requires a 5–10% CO₂ environment for maintenance of physiological pH. VitaCellix™ offers a variety of DMEM/F12 modifications for a range of cell culture applications— find your DMEM/F12 media here.